Like you, our valued clients and partners, we are scientists, and for over 30 years our expertise has been committed to the discovery of new high-end analytical methods and investigation means. Immunoassays, fluorescence, and biology are all in our DNA. Working side by side with you, we are able to create the tools that will deliver rapid and accurate results with total confidence.
In biology, like in scientific relationships, interaction is everything!
HTRF Assay Principle
HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. For a sandwich assay, two antibodies that recognize a protein of interest are used, with one antibody coupled to a donor, and the other with the acceptor. If the 2 antibodies recognize the analyte, the donor will emit fluorescence upon excitation and the energy will be transferred to the nearby acceptor, giving specific acceptor fluorescence. The donor fluorescence is also measured and a ratio with the acceptor fluorescence is applied.
HTRF®: the gold reference for time-resolved FRET
HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn’s Nobel prize-winning work on rare earth complexes. Due to the chemical and photophysical characteristics of these fluorescent dyes, HTRF exhibits performance levels in stability and specificity that make it unparalleled for time-resolved FRET studies. It is especially extremely stable in time, allowing repetitive measurements for days. The very structure of HTRF reagents also makes assays highly resistant to most experimental conditions, including additives, chelates, DMSO, ionic strength, challenging cations, pH, temperature, or cell culture media. See the reagent chemistry tab for more details about HTRF reagents.
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behavior or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.